Efficacy of Trichoderma strains against Fusarium oxysporum f. sp ciceri, the Incitant of Wilt Disease in Chickpea

摘要

Thirty isolates of Trichoderma belonging to three species - T. harzianum, T. viride and T. virens collected from diverse agro-ecological habitat were evaluated against Fusarium oxysporum f.sp. ciceri (Foc) Kanpur isolate to test their antagonistic potential in chickpea. Of the 30 isolates of Trichoderma, isolates IPT 2, 4, 6, 8, 10, 16, 17, 22, 25, 28 and 29 were found promising in reducing mycelial growth and conidia production of in dual cultures. Non-volatile compounds of the culture filtrate of Trichoderma isolates (IPT 5, 7, 11, 13, 14, 15, 16, 17, 19,25 and 31) were highly detrimental to growth and sporulation of (85.7 - 100% inhibition). Similarly, volatile metabolites produced by Trichoderma isolates (IPT 2, 6, 9, 11, 16, 20, 24, 28 and 31) reduced growth by 25.0-65.3 % and conidia production by 25.0-87.5% of. were highly effective. In seed inoculated plants, there was a significant decrease in wilt incidence caused by. Both in dry and wet seed treatments Trichoderma isolates IPT 2, 4, 6, 8, 11, 19, and 31 effectively reduced the wilt incidence by 23.0 to 53.5% compared to control, but without any significant difference among the two methods of seed treatment.