Enantioselective epoxide hydrolase activity of a newly isolated microorganism,Sphingomonas echinoides EH-983,from seawater

摘要

A marine microorganism,Sphingomonas echinoides EH-983,which possesses epoxide hydrolase(EH)activity was isolated from seawater and characterized.The EH of S.echinoides EH-983 preferentially metabolized(R)-enantiomer when the racemic styrene oxides were supplied as substrates.The optimal pH and temperature for the enantioselective hydrolysis by whole-cells of S.echinoides EH-983 were 7.0 and 20 deg C,respectively.When kinetic resolution was conducted with a racemic mixture of styrene oxides at an initial concentration of 40 mM,enantiopure(S)-styrene oxide was obtained in 180min with a yield of 21.3%.To our best knowledge,S.echinoides EH-983 is the first marine microorganism that is reported to have EH activity.