Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis

Motre Aurelie; Kong Richard; Li Ying

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Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis

机译：提高等温DNA扩增速度以快速检测结核分枝杆菌

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In this study, we report the development of a helicase-dependent amplification assay for rapid detection of Mycobacterium tuberculosis (MTB). By applying a step-by-step optimization method, the amplification time from an input of 2-copy MTB genomic DNA was reduced from about 60min to less than 30min.

机译：在这项研究中，我们报告了快速检测结核分枝杆菌（MTB）的解旋酶依赖性扩增方法的开发。通过应用逐步优化方法，输入2拷贝MTB基因组DNA的扩增时间从大约60分钟减少到不到30分钟。

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《Journal of Microbiological Methods 》 |2011年第2期| 共3页
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Motre Aurelie; Kong Richard; Li Ying;
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正文语种 eng
中图分类微生物研究与微生物实验 ;
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1. Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis [J] . Motre Aurelie, Kong Richard, Li Ying Journal of Microbiological Methods . 2011 ,第2期

机译：提高等温DNA扩增速度以快速检测结核分枝杆菌
2. Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes [J] . Rafati Adele, Gill Pooria Mikrochimica Acta: An International Journal for Physical and Chemical Methods of Analysis . 2015 ,第3a4期

机译：微流控方法在毛细管中使用环介导的等温扩增快速比浊法检测结核分枝杆菌的DNA
3. Use of loop-mediated isothermal amplification of DNA for the rapid detection of Mycobacterium tuberculosis in clinical specimens. [J] . Neonakis IK, Spandidos DA, Petinaki E European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology . 2011 ,第8期

机译：使用环介导的DNA等温扩增技术快速检测临床标本中的结核分枝杆菌。
4. Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick biosensor [C] . Kaewphinit Thongchai, Santiwatanakul Somchai, Jaratsing Pronpan, Biomedical Engineering International Conference (BMEiCON), 2011 . 2011

机译：环介导的等温扩增结合侧向量油尺生物传感器检测结核分枝杆菌
5. Development of a bio-nanoparticle DNA-based biosensor for tuberculosis detection using isothermal amplification. [D] . Torres-Chavolla, Edith. 2011

机译：基于等温扩增的用于结核病检测的基于生物纳米粒子DNA的生物传感器的开发。
6. Multiple strategies to improve sensitivity speed and robustness of isothermal nucleic acid amplification for rapid pathogen detection [O] . Yanhong Tong, Bertrand Lemieux, Huimin Kong 2011

机译：多种策略可提高等温核酸扩增的灵敏度速度和鲁棒性以快速检测病原体
7. Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes [O] . Rafati A., Gill P. 2015

机译：微流控方法在毛细管中使用环介导的等温扩增快速比浊法检测结核分枝杆菌的DNA

Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis

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