Microsomal cytochrome P450 2C5: comparison to microbial P450s and unique features

摘要

Although microsomal P450s represent the majority of P450s, only microbial P450s have been amenable to crystal structure solution. We have recently solved the first crystal structure of a microsomal P450, 2C5, a progesterone hydroxylase from rabbit. We dscuss the features of the protein in common with existing structures of microbial P450s and limitations of homology modeling mammaian P450s based on the microbial structures. Unique features involving membrane, substrate and cytochrome P450 reductase interaction are also discussed.