SHOOT MULTIPLICATION AND PLANT REGENERATION OF GUAVA (Psidium guajava L.) FROM NODAL EXPLANTS OF IN VITRO RAISED PLANTLETS

摘要

A micropropagation method is described for guava (Psidium guajava L.) using nodal explants; from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N-6-benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (IAA) or alpha-naphthalene acetic acid (NAA). Medium containing 1 mg l(-1) BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (IBA). The rooted plantlets were successfully acclimatized.