首页> 外文期刊>Journal of industrial microbiology & biotechnology >Whole-genome sequencing of Bacillus subtilis XF-1 reveals mechanisms for biological control and multiple beneficial properties in plants
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Whole-genome sequencing of Bacillus subtilis XF-1 reveals mechanisms for biological control and multiple beneficial properties in plants

机译:枯草芽孢杆菌XF-1的全基因组测序揭示了生物防治机制和植物的多种有益特性

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Bacillus subtilis XF-1 is a gram-positive, plant-associated bacterium that stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. In particular, it is especially highly efficient at controlling the clubroot disease of cruciferous crops. Its 4,061,186-bp genome contains an estimated 3853 protein-coding sequences and the 1155 genes of XF-1 are present in most genome-sequenced Bacillus strains: 3757 genes in B. subtilis 168, and 1164 in B. amyloliquefaciens FZB42. Analysis using the Cluster of Orthologous Groups database of proteins shows that 60 genes control bacterial mobility, 221 genes are related to cell wall and membrane biosynthesis, and more than 112 are genes associated with secondary metabolites. In addition, the genes contributed to the strain's plant colonization, bio-control and stimulation of plant growth. Sequencing of the genome is a fundamental step for developing a desired strain to serve as an efficient biological control agent and plant growth stimulator. Similar to other members of the taxon, XF-1 has a genome that contains giant gene clusters for the non-ribosomal synthesis of antifungal lipopeptides (surfactin and fengycin), the polyketides (macrolactin and bacillaene), the siderophore bacillibactin, and the dipeptide bacilysin. There are two synthesis pathways for volatile growth-promoting compounds. The expression of biosynthesized antibiotic peptides in XF-1 was revealed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
机译:枯草芽孢杆菌XF-1是革兰氏阳性植物相关细菌,可刺激植物生长并产生抑制土壤传播的植物病原体的次级代谢产物。特别是,它在控制十字花科作物的根瘤病方面特别高效。它的4,061,186-bp基因组包含估计的3853个蛋白编码序列,XF-1的1155个基因存在于大多数基因组测序的芽孢杆菌菌株中:枯草芽孢杆菌168中有3757个基因,解淀粉芽孢杆菌FZB42中有1164个基因。使用直系同源簇蛋白质数据库的分析显示,有60个基因控制细菌的活动,其中221个基因与细胞壁和膜的生物合成有关,而超过112个基因与次生代谢产物相关。此外,这些基因还有助于菌株的定植,生物控制和刺激植物生长。基因组测序是开发所需菌株以用作有效的生物防治剂和植物生长刺激剂的基本步骤。与分类单元的其他成员相似,XF-1的基因组包含巨大的基因簇,可用于非核糖体合成抗真菌脂肽(表面活性素和丰霉素),聚酮化合物(大环内酯和杆菌素),铁载体细菌杆菌素和二肽杆菌素。挥发性促进生长的化合物有两种合成途径。基质辅助激光解吸/电离飞行时间质谱揭示了XF-1中生物合成的抗生素肽的表达。

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