Preparation and characterization of the inclusion complex of Ofloxacin with β-CD and HP-β-CD

摘要

The formation of the inclusion complexes of Ofloxacin with cyclodextrins (CDs) including β-cyclo-dextrin (β-CD), and hydroxypropyl-β-cyclodextrin (HP-β-CD) were studied by Fluorescence, UV-Vis absorption spectroscopy and nuclear magnetic resonance spectroscopy (NMR) in solution. Experimental conditions including the concentration of various CDs and media acidity were investigated in detail at room temperature. The results suggested that in different pH solutions, CDs have different inclusive capacity to different forms Ofloxacin.β-CD was most suitable for inclusion of neutral form and HP-β-CD was suitable for acidic form. The binding constant (K) of the inclusion complex was determined by fluorescence measurement, and the complexation ratio was determined as 1:1 in the concentration range used in this study. A mechanism was proposed to explain the inclusion process based on the experimental NMR data.