Identification and Quantification of Phenolic Compounds in Alpinia galanga and Alpiniacalcarata and its Relation to Free Radical Quenching Properties: A Comparative Study

摘要

The bio-active phenolic compounds in Alpinia galanga and A. calcarata were characterized by high-performance liquid chromatography coupled with mass spectroscopy method. LC-MS/MS analysis showed that gallic acid and ellagic acid were the major phenolic compounds in A. galanga (320 and 518 mg.kg~(-1), respectively) and A. calcarata (75 and 1,200 mg.kg~(-1), respectively). The method was validated for its linearity, precision, and accuracy as well as sensitivity. A. galanga showed better free radical scavenging activity against DPPH and ABTS* with an IC_(50 )value of 21.26 and 25.54 |x g.mlr~(-1), respectively than A. calcarata (56.51 and 62.35 |x g.mlr~(-1), respectively). The antioxidant activity of A. galanga may be due to higher amounts of phenolic compounds present in this plant.