FGF19 protects colonic epithelial cells against hydrogen peroxide.

摘要

BACKGROUND/AIMS: The apoptosis induced by hydrogen peroxide (HO) in colonic epithelial cells is very important for the pathogenesis of inflammatory bowel disease (IBD). Fibroblast growth factor (FGF) 15, the human ortholog of FGF19, is reported to be secreted from colonic myofibroblasts and enhances colonic epithelial restitution, but little is known about the function of FGF19 to colonic epithelial cells. In the present study, we investigate the anti-apoptosis effect of FGF19 in colonic epithelial cells treated with HO. METHODS: Young adult mouse colonic epithelial (YAMC) cells are used to investigate the protective role of FGF19. Cellular viability was determined by WST-8 assay, and apoptosis was measured by Hoechst staining and Western blotting of cleaved caspase-3. YAMC cells were pretreated by FGF19 and HO was used for cellular damage. RESULTS: We demonstrated that pretreatment of FGF19 (50 ng/ml) significantly protects YAMC cells treated with HO assessed by WST-8. We also demonstrated Hoechst staining of YAMC cells and that HO-induced apoptosis is significantly reduced by FGF19 treatment via inhibition of the caspase-3 pathway. CONCLUSION: These results indicate FGF19 protects YAMC cells against HO and might be related to the pathogenesis of IBD. Even further studies are needed - FGF19 may be one of the possible therapeutic strategies of IBD.