Spectrofluorometric and high-performance liquid chromatographic determination of all-rac- alpha -tocopheryl acetate in virgin olive oil.

摘要

A high-performance liquid chromatographic (HPLC) method was developed for the analysis of all-rac- alpha -tocopheryl acetate in olive oil. After extraction of all-rac- alpha -tocopheryl acetate from olive oil into hexane/ethyl acetate (4:1, v/v), it was measured with HPLC-diode array detection and regular external standard calibration and confirmed with liquid chromatography-mass spectrometry. Spectrofluorometric and standard addition methods were also used to quantify all-rac- alpha -tocopheryl acetate in olive oil. A standard addition method is based on fortification of known concentrations of all-rac- alpha -tocopheryl acetate in a sample. The concentrations (30.4+or-1.2 micro g/mL) of all-rac- alpha -tocopheryl acetate determined with the HPLC method were comparable with those (32.6+or-1.1 micro g/mL) with the spectrofluorometric method. Standards were checked for linearity giving correlation coefficients of higher than 0.999 in a concentration range from 0.5 to 20 micro g/mL.