Prevalence and Antibiotic Resistance of Salmonella SPP. and Salmonella Typhimurium in Broiler Carcasses Wings and Liver

摘要

The objectives of this study were to isolate and identify Salmonella spp. from broiler carcasses, wings and liver samples by immunomagnetic separation based cultivation technique, to verify the isolates as Salmonella spp. by the detection of oriC gene by PCR, to identify the isolates using malic acid dehydrogenase and DT104 specific primers as S. Typhimurium and S. Typhimurium DT104. Also to determine the two important virulence genes, virulence plasmid (spvC) and invasion (invA) for molecular characterization, to evaluate the antibiotic resistance profiles of the isolates. For this purpose, 110 broiler carcasses, 110 broiler wings and 110 broiler liver samples with a total number of 330 were analyzed. Ninety six (29.1%) of the samples were detected as contaminated with Salmonella spp. According to the results 11 isolates (11.4%) were identified as S. Typhimurium. None of these serotypes were determined as specific phage type DT104. InvA gene was detected from all the (100.0%) Salmonella isolates and 14 isolates (14.6%) were detected as positive for spvC gene. Eighty-three isolates (86.4%) were resistant to at least 5, 70 isolates (72.9 %) resistant to at least 7 and 36 isolates (37.5%) were resistant to at least 9 antibiotic.