Genetic diversity and a heterogeneous population of Citrus mosaic virus within a single citrus tree

摘要

Plural variants of Citrus mosaic virus (CiMV), a Satsuma dwarf virus (SDV) strain, were detected from a single citrus tree. Reverse transcription-polymerase chain reaction amplification of 1139 nucleotides (nt) of 3'-terminal RNA1 and analyses of nucleotide sequences revealed three CiMV variants that differed by up to 5.4%. The analyses of 1403-1493 nt of 3'-terminal RNA2 showed fourCiMV variants, two of which differed in the presence of an 89-nt insert related to a 29- to 30-nt repeat in the 3'-noncoding regions. This is the first report to show multiple infections by SDV variants that can be generated by genetic mutations.