Effect of soybean oligosaccharides on immunity and TLR2-NF- kappa B signal pathway response for weanling pigs.

摘要

Weaning body leads to intestinal immunological stress and decreases nutrient digestion, absorption and metabolism. We tried to modulate immunological stress with oligosaccharide and elucidate the signal pathway. This study was conducted to investigate the effects of SBOS on plasma concentration of immunoglobulin and interleukin and TLR2-NF- kappa B signal pathway in weanling mini-piglets. Twelve piglets weaned at 21 day of age were randomly assigned into 0 or 0.5 g/kg of SBOS-supplemented group (6 piglets per group), respectively. At the end of a 14-day feeding period, concentrations of IgG, IgM and IL-2 in plasma and mRNA expression levels of TLR2, MyD88 and NF- kappa B p65 were measured. Results showed that the SBOS supplementation increased concentrations of immunoglobulins IgG (P<0.05) and IgM (P>0.05) in plasma, as well as cytokines (IL-2) (P<0.05), when compared with the control group; the expression level of TLR2 and MyD88 in the spleen, and of NF- kappa B p65 and MyD88 in the colon of the piglets fed SBOS-supplemented diet were lower (P<0.05), while NF- kappa Bp65 in the spleen and TLR2 in the colon had no significant difference in comparison with the control group. We conclude that SBOS influences intestinal and spleen immunity by TLR2-NF- kappa B signal pathway in weanling pigs.