Detection methods for Shiga-like toxin producing Escherichia coli O157:H7 from foods of animal origin: a review.

摘要

Escherichia coli O157:H7 has emerged as one of the important contemporary human food borne pathogen capable of inflicting various disease symptoms in man. Main reservoir of this pathogen in nature is cattle and the food products made from them such as milk, meat and their products. It causes colitis and bloody diarrhea by producing a toxin called Shiga toxin, which damages the intestines. The approach for the diagnosis of this infection generally depends upon the usage of biochemical characteristics, nucleic acid based techniques like DNA probes, PCR and immunoblotting with antibodies to verotoxin. This review discusses in detail various methods, which are available for the detection of this deadliest pathogen from animal foodstuffs including the conventional microbiological and newer molecular approaches.