Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila.

摘要

A multiplex polymerase chain reaction (m-PCR) method developed to rapidly and specifically detect potentially pathogenic A. hydrophila strains is described. 30 experimentally infected Carassius auratus gibelio were used to test the diagnostic method, which amplified the 16S ribosomal DNA and aerolysin genes of A. hydrophila. The results were compared with that of a Dot-ELISA method. It was shown that the m-PCR method identified potential pathogenic A. hydrophila strains in <8 h. The coincident rate of detection of m-PCR and Dot-ELISA was 94.4%. The detection rates obtained by m-PCR and Dot-ELISA were 18/30 (60%) and 12/30 (40%), respectively. In conclusion, the m-PCR method is useful for the direct detection of pathogenic strains of A. hydrophila, and is also specific, less costly and easier to use compared to biochemical and DNA hybridization methods..