首页> 外文期刊>Journal of Experimental Botany >Genotype effects on ABA consumption and somatic embryo maturation in interior spruce (Picea glauca x engelmanni).
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Genotype effects on ABA consumption and somatic embryo maturation in interior spruce (Picea glauca x engelmanni).

机译:基因型对内在云杉(Picea glauca x engelmanni)中ABA消耗和体细胞胚成熟的影响。

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摘要

Abscisic acid (ABA) plays an important role during somatic embryo development and maturation in coniferous species. The purpose of this research was to study ABA utilization by genotypes with different embryo maturation capabilities in interior spruce. Cell lines ISP11 and ISP48 were of high embryo maturation capability. By contrast, the tissue of line ISP16 contained numerous immature embryos, but only a few mature embryos developed. Exogenous ABA, i.e. S-ABA [(+)-cis, trans-ABA], racemic ABA, or ABA isomers were added into suspension cultures at a final concentration of 30 micro M. In comparison to racemic ABA and ABA isomers, S-ABA reduced tissue proliferation the most. In all cell lines, about half of the racemic ABA was used within 2 weeks; the remaining ABA was (-)-cis, trans-ABA. The concentration of ABA showed little change thereafter. In the cultures supplied with ABA isomers, about half of (+/-)-cis, trans-ABA was utilized during 22 d. By contrast, (+/-)-trans, trans-ABA was hardly used, especially in line ISP16. S-ABA was almost completely metabolized by line ISP11. However, approximately 28% and 22% of the S-ABA remained in the culture of cell lines ISP16 and ISP48, respectively. Cell line ISP16 grew the fastest in culture. By 3 weeks, S-ABA consumption by ISP11 and ISP48 on the basis of tissue growth was, respectively, 2.2-fold and 3.4-fold greater than that of ISP16. A higher ratio of dihydrophaseic acid to phaseic acid existed with cell lines of higher embryo maturation capability, especially when the exogenously supplied ABA was chemically synthesized..
机译:脱落酸(ABA)在针叶树种的体细胞胚发育和成熟过程中起着重要作用。这项研究的目的是研究内部云杉中具有不同胚胎成熟能力的基因型对ABA的利用。 ISP11和ISP48细胞系具有很高的胚胎成熟能力。相比之下,ISP16系的组织包含许多未成熟的胚胎,但只发育了一些成熟的胚胎。将外源ABA,即S-ABA [(+)-顺式,反式ABA],外消旋ABA或ABA异构体添加到悬浮培养物中,最终浓度为30 microM。与外消旋ABA和ABA异构体相比,S- ABA最大程度地减少了组织增殖。在所有细胞系中,在2周内使用了约一半的外消旋ABA。其余的ABA是(-)-顺式,反式ABA。此后,ABA的浓度几乎没有变化。在提供ABA异构体的培养物中,在22 d内利用了大约一半的(+/-)-顺式反式ABA。相反,几乎不使用(+/-)-trans,trans-ABA,特别是在ISP16中。 ISP11系几乎完全代谢了S-ABA。然而,分别在细胞系ISP16和ISP48的培养物中保留了约28%和22%的S-ABA。 ISP16细胞系在培养中增长最快。到3周时,基于组织生长的ISP11和ISP48消耗的S-ABA分别是ISP16的2.2倍和3.4倍。胚胎成熟能力较高的细胞系中存在较高的二氢相酸与相酸的比例,尤其是在化学合成外源提供的ABA时。

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