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The growth limits of a large number of Listeria monocytogenes strains at combinations of stresses show serotype- and niche-specific traits

机译:在压力组合下大量单核细胞增生李斯特菌菌株的生长极限表现出血清型和生态位特异性

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The aim of this study was to associate the growth limits of Listeria monocytogenes during exposure to combined stresses with specific serotypes or origins of isolation, and identify potential genetic markers. The growth of 138 strains was assessed at different temperatures using combinations of low pH, sodium lactate, and high salt concentrations in brain heart infusion broth. None of the strains was able to grow at pH [less-than or equal to] 4p"4, aw [less-than or equal to] 0p"92, or pH [less-than or equal to] 5p"0 combined with aw [less-than or equal to] 0p"94. In addition, none of the strains grew at pH [less-than or equal to] 5p"2 and NaLac greater-than-or-equal 2%. At 30pC, the serotype 4b strains showed the highest tolerance to low pH and high NaCl concentrations at both pH neutral (pH 7p"4) and mild acidic conditions (pH 5p"5). At 7pC, the serotype 1/2b strains showed the highest tolerance to high NaCl concentrations at both pH 7p"4 and 5p"5. Serotype 1/2b meat isolates showed the highest tolerance to low pH in the presence of 2% sodium lactate at 7pC. ORF2110 and gadD1T1 were identified as potential biomarkers for phenotypic differences. Differences in growth limits were identified between specific L. monocytogenes strains and serotypes, which could in some cases be associated with specific genetic markers. Our data confirm the growth limits of L. monocytogenes as set out by the European Union for ready-to-eat foods and provides an additional criterion. The association of L. monocytogenes serotypes with certain stress responses might explain the abundance of certain serotypes in retail foods while others are common in clinical cases.
机译:这项研究的目的是将单核细胞增生性李斯特菌的生长极限与特定的血清型或分离起源联系起来,并确定潜在的遗传标记。使用低pH,乳酸钠和脑心输液肉汤中高盐浓度的组合,在不同温度下评估了138株菌株的生长。没有一个菌株能够在pH [小于或等于] 4p“ 4,aw [小于或等于] 0p” 92或pH [小于或等于] 5p“ 0的条件下生长。 aw [小于或等于] 0p“ 94。此外,没有一个菌株在pH [小于或等于] 5p“ 2和NaLac大于或等于2%的条件下生长。在30pC时,血清型4b菌株显示出对低pH和高NaCl的最高耐受性在pH中性(pH 7p“ 4)和弱酸性条件(pH 5p” 5)时,血清型1 / 2b菌株对pH 7p“ 4和5p” 5的高NaCl浓度表现出最高的耐受性。血清型1 / 2b肉分离物在2%的乳酸钠存在下于7pC表现出对低pH的最高耐受性.ORF2110和gadD1T1被鉴定为潜在的表型差异生物标志物。 ,在某些情况下可能与特定的遗传标记有关我们的数据证实了欧盟规定的即食食品单核细胞增生李斯特菌的生长极限,并提供了附加标准。某些压力反应可能会普通零售食品中某些血清型的丰富性,而其他血清型在临床病例中很常见。

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