Improvement of Candida sake biocontrol activity against post-harvest decay by the addition of ammonium molybdate

摘要

Aim: To improve the efficacy of Candida sake by adding ammonium molybdate to control post-harvest decay in Golden Delicious apples. Methods and Results: In laboratory trials, C. sake 2 * 10~6 cfu ml~(-1) plus 5 mmol l~(-1) ammonium molybdate greatly reduced Penicillium expansum, Botrytis cinerea or Rhizopus stolonifer in apples stored at 20 ℃ for 7 days, and reduced by more than 90% blue and grey mould in apples stored at 1 ℃ for 60 days. The consistency of these results was maintained in semi-commercial trials at 1 ℃ in air and in a low oxygen atmosphere for 120 days. The pre-harvest application of C. sake 2 * 10~7 cfu ml~(-1) plus l mmol l~(-1) ammonium molybdate did not improve post-harvest biocontrol of blue mould. The population of C. sake significantly decreased in the presence of ammonium molybdate in apple wounds. conclusion: The addition of ammonium molybdate at 5 mmol l~(-1) to C. sake enhanced the efficacy of the antagonist to control post-harvest diseases on applies. Significance and Impact of the Study: Ammonium molybdate significantly reduces the amount of C. sake biomass required to achieve post-harvest disease control, with a consequent reduction in costs. This may be useful in the industrial production of C. sake.