Production of Aspergillus xylanase by lignocellulosic waste fermentation and its application

摘要

Strains of Aspergillus terreus and A. niger, known to produce xylanase with undetectable amounts of cellulase, were studied for xylanase (EC 3.2.1.8) production on various lignocellulosic substrates using solid state fermentation. Of the lignocellulosic substrates used, wheat bran was the best for xylanase production. The effects of various parameters, such as moistening agent, level of initial moisture content, temperature of incubation, inoculum size and incubation time, on xylanase production were studied. The best medium for A. terreus was wheat bran moistened with 1:5 Mandels and Strenberg mineral solution containing 0 centre dot 1% tryptone, at 35 det C, and at inoculum concentration 2 * 10~7 - 2 * 10~8 spores 5 g~(-1) substrate; for A. niger, the best medium was wheat bran moistened with 1:5 Mandels and Strenberg mineral solution containing 0 centre dot 1% yeast extract, at 35 deg C, and at an inoculum concentration of 2 * 10~7 - 2 * 10~8 spores 5 g~(-1) substrate. Under these conditions, A. terreus produced 68.9 IU ml~(-1) of xylanase, and A. niger, 74.5 IU ml~(-1), after 4 d of incubation. A crude culture filtrate of the two Aspergillus strains was used for the hydrolysis of various lignocellulosic materials. Xylanase preparations from the two strains selectively removed the hemicellulose fraction from all lignocellulosic materials tested.