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mutienzymkomposition with glucoamylase, protease activity and xylanase, and procedures to production by aspergillus fermentation in solid getreideweizenmedium

机译:葡糖淀粉酶的突变酶固定,蛋白酶活性和木聚糖酶,以及在固体getreideweizenmedium中曲霉菌发酵生产的程序

摘要

Enzyme preparation (I) produced by fermenting wheat bran with an Aspergillus niger strain has glucoamylase, protease and xylanase activities of at least 100 U/g (dry basis), provided that the glucoamylase activity is at least 750 U/g and/or the xylanase activity is at least 300 U/g. An Independent claim is also included for the production of (I) by: (1) moistening and pasteurizing or sterilizing wheat bran; (2) inoculating an at least 10 cm thick layer of the bran with Aspergillus niger; and (3) fermenting the bran in an aerated and periodically agitated reactor at 28 - 38 degrees C for 1 - 3 days while maintaining the moisture content of the bran at 50 - 60 wt.%.
机译:通过用黑曲霉菌株发酵麦麸生产的酶制剂(I)的葡糖淀粉酶,蛋白酶和木聚糖酶活性至少为100 U / g(干基),条件是葡糖淀粉酶活性至少为750 U / g和/或木聚糖酶活性为至少300U / g。对于(I)的生产,还包括以下独立权利要求:(1)对麦麸进行润湿和巴氏杀菌或灭菌; (2)用黑曲霉接种至少10厘米厚的麸皮层; (3)将麸皮在28-38℃的充气且定期搅拌的反应器中发酵1-3天,同时将麸皮的水分含量保持在50-60wt。%。

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