Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases

Fachmann M. S. R.; Josefsen M. H.; Hoorfar J.; Nielsen M. T.; Lofstrom C.

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Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases

机译：具有抑制性的DNA聚合酶对弯曲杆菌和沙门氏菌进行基于PCR的实时检测的经济高效优化

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AimsThe aim of this study was to cost-effectively improve detection of foodborne pathogens in PCR inhibitory samples through the use of alternative DNA polymerases.

机译：目的本研究的目的是通过使用替代性DNA聚合酶来经济高效地改善PCR抑制性样品中食源性病原体的检测。

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《Journal of applied microbiology》 |2015年第5期|共12页
作者
Fachmann M. S. R.; Josefsen M. H.; Hoorfar J.; Nielsen M. T.; Lofstrom C.;
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正文语种 eng
中图分类应用微生物学;
关键词
Campylobacter; detection; DNA polymerase; faeces; inhibitors; minced meat; Salmonella; sample preparation;

机译：弯曲杆菌;检测;DNA聚合酶;粪便;抑制剂;肉末;沙门氏菌;样品制备;

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1. Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases [J] . Fachmann M. S. R., Josefsen M. H., Hoorfar J., Journal of applied microbiology . 2015,第5期

机译：具有抑制性的DNA聚合酶对弯曲杆菌和沙门氏菌进行基于PCR的实时检测的经济高效优化
2. Detection of Salmonella spp., Yersinia enterocolitica, Listeria monocytogenes and Campylobacter spp. by real-time multiplex PCR using amplicon DNA melting analysis and probe-based assay [J] . Jurate Skerniskyte, Julija Armalyte, Raimonda Kvietkauskaite, International journal of food science & technology . 2016,第2期

机译：检测沙门氏菌，小肠结肠炎耶尔森菌，单核细胞增生性李斯特菌和弯曲杆菌。通过实时多重PCR，使用扩增子DNA熔解分析和基于探针的分析
3. Optimization of rapid Salmonella enterica detection in liquid whole eggs by SYBR green I-Based real-time reverse transcriptase-polymerase chain reaction. [J] . Techathuvanan C, DSouza DH Foodborne pathogens and disease . 2011,第4期

机译：通过基于SYBR green I的实时逆转录酶-聚合酶链反应快速优化液体全蛋中沙门氏菌的检测。
4. APPLICATION OF SYBR GREEN REAL-TIME POLYMERASE CHAIN REACTION FOR SIMULTANEOUS DETECTION OF CAMPYLOBACTER JEJUNI, ESCHERICHIA COLI O157:H7 AND SALMONELLA SPP [C] . H.M. Nam, B.E. Gillespie, S.E. Murinda, Annual Meeting of the National Mastitis Council . 2004

机译：SYBR绿色实时聚合酶链反应在同时检测云静电杆菌，大肠杆菌O157：H7和沙门氏菌SPP的应用
5. Development of a rapid detection method of Salmonella spp. on chicken skin by real-time polymerase chain reaction. [D] . Carter, Melvin. 2008

机译：沙门氏菌快速检测方法的开发。通过实时聚合酶链反应在鸡皮上
6. Optimization of a 12-Hour TaqMan PCR-Based Method for Detection of Salmonella Bacteria in Meat [O] . M. H. Josefsen, M. Krause, F. Hansen, 1979

机译：基于12小时TaqMan PCR的肉类沙门氏菌检测方法的优化
7. Cost-effective optimization of real-time PCR based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases [O] . Fachmann, Mette Sofie Rousing, Josefsen, Mathilde Hasseldam, Hoorfar, Jeffrey, 2015

机译：基于实时pCR的弯曲杆菌和沙门氏菌检测耐受性DNa聚合酶的成本效益优化

Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases

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