Effect of auxins, cytokinin, gibberellin and kind of sugar on callus induction in ovules of Gossypium hirsutum X Gossypium arboreum crosses

摘要

For the ovule-callus culture studies in crosses between G. hirsutum and G. arboreun cotton, the immature hybrid embryos of four crosses viz. H 777 X HD 107, H 777 X HD 123, HS 6 X HD 107 and HS 6 X HD 123 excised 3 days after pollination (DAP) were used. They were cultured on MS media supplemented with various concentrations and combinations of auxins, cytokinins, gibberelins and kind of sugars. Normally in many crosses, especially between tetraploids and diploids, the hybrid embryos abort at a veryearly stage. The early abortion of the embryo was prevented by treatment of the flowers immediately after pollination with a solution of gibberellic acid and naphathalene acetic acid and when grown to a stage where the embryo is few celled, the ovules excised and cultured on media to form calli. The maximum response was observed when MS media was supplemented with IAA (1.0 mg/l). Kin (0.2 mg/l). Ch (250 ml) and sucrose (3 percent).