Molecular characterization of two new Cu/Zn superoxide dismutase genes from halophyte Aeluropus lagopoides.

摘要

High-level reactive oxygen species reduce plant growth and development. Plants use different systems to mitigate such effects. Superoxide dismutase (SOD) is one of the most important antioxidants, which detoxifies superoxide radicals. The expression of SOD enzymes increases under stress conditions in salt- and drought-tolerant plants. Because SOD plays a critical role in plants' tolerance to stressful conditions, we provide molecular characterization of SOD genes. In this study, extraction of RNA and construction of cDNA were done for gene amplification from halophyte plant Aeluropus lagopoides. Two SOD genes were isolated and cloned in Escherichia coli. Then the genes were sequenced. Two novel SOD genes, AlSOD1 and AlSOD2, have been deposited in Genebank under accession # HM467789 and HM536180.1. The result of bioinformatics analysis indicated the genes were complete and open reading frames encoded 152 amino acids in both SOD proteins. Molecular weight and isoelectric points, respectively, were 15.097 and 5.65 for AlSOD1 and 19.387 and 5.15 for AlSOD2. The present study can be useful in crop breeding programs aimed at developing plants tolerant to salt and drought stress.