Quantitative liquid chromatographic and tandem mass spectrometric determination of vitamin D3 in human serum with derivatization: A comparison of in-tube LLE, 96-well plate LLE and in-tip SPME

摘要

Sensitive and selective methods based on high performance liquid chromatography (HPLC) with tandem mass spectrometric (MS/MS) detection were developed for the determination of vitamin D3 in human serum. Derivatization of vitamin D3 and its stable isotope labeled internal standard provided highly sensitive quantification and selective detection from endogenous compounds. Samples were prepared using the in-tube liquid-liquid extraction (LLE), 96-well plate LLE, and in-tip solid phase micro-extraction (SPME) in 96-well format. In all methods, the MS/MS detection was performed using Applied Biosystems-Sciex API 3000 tandem mass spectrometers interfaced with a heated nebulizer probe and operated in the positive ionization mode. Both tube and plate LLE methods achieved a lower limit of quantitation (LLOQ) of 0.5ng/mL when 1.0 and 0.4mL of human serum was processed, respectively, and were validated in the concentration range of 0.5-25ng/mL; while for the in-tip SPME method, LLOQ was 5ng/mL with only 0.1mL of human serum required. Comparisons were made among three different methods, including precision and accuracy, sample throughput, recovery and matrix effects.