Development and validation of a high performance liquid chromatography assay for 17 alpha-methyltestosterone in fish feed

摘要

17 alpha-Methyltestosterone (MT) is used to manipulate the gender of a variety of fish species. A high performance liquid chromatography (HPLC) internal standard method for the determination of 17 alpha-methyltestosterone in fish feed using 3 beta-methoxy-17 beta-hydroxyandrost-5-en-7-one as internal standard (IS) has been developed. The method has been validated for the quantitation of MT in fish feed using 245 nm UV absorbance as the parent wavelength and 255 nm as a qualifier wavelength. The method was validated in the concentration range of 15.0-120 mg/kg of 17 alpha-methyltestosterone in fish feed. Method was also found to be suitable for other feeds. (c) 2005 Elsevier B.V. All rights reserved.