采用AcclaimTMSurfactant Plus色谱柱(4.6 mm×250 mm×5 μm)和紫外检测器,研究了测定甲基丙烯酰氧乙基三甲基氯化铵(DMC)含量的高效液相色谱分析方法,流动相为0.1 mol/L的乙酸铵溶液-乙腈(用乙酸调至pH至5.00),检测波长为210 nm、柱温为30 ℃.实验结果表明,在50~250 mg/L范围内,DMC的质量浓度与峰面积呈现良好的线性关系,相关系数为0.9994,平均加标回收率为91.3%~108.4%,相对标准偏差为0.25%~0.34%,检出限为0.0505 mg/L.该方法快速、准确、重现性高,可用于科研及生产领域中DMC的定性定量分析.%A high performance liquid chromatographic method for determining the content of methacryloyloxyethyl trimethyl ammonium chloride(DMC) was studied using an AcclaimTMSurfactant Plus column(4.6 mm×250 mm×5 μm) and a UV detector,with a mobile phase being 0.1 mol/L ammonium acetate-acetonitrile(pH 5.00,adjusted with acetic acid),a detection wavelength being 210 nm and a column temperature being 30 ℃. The experimental results indicated that a good linear relationship was obtained between the peak area and the mass concentration of DMC in the range of 50-250 mg/L,with a correlation coefficient being 0.999 4,an average standard recovery ranging from 91.3% to 108.4% and a relative standard deviation ranging from 0.25% to 0.34% and a limit of detection being 0.050 5 mg/L. The method is fast,accurate,high in reproducibility and suitable for qualitative and quantitative analysis of DMC in the fields of scientific research and production.
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