Analysis of cyanolipids and triacylglycerols from sapindaceae seed oils with high-temperature gas chromatography and high-temperature gas chromatography-chemical ionization mass spectrometry

摘要

The seed oils of three Sapindaceae species have been examined by high-temperature gas chromatography using glass capillary columns coated with the stationary phases SOP-50 (50% diphenyl-50% dimethylpolysiloxane, methoxy-termi-nated) and OV-225-OH (25%phenylmethyl-25% cyanopropylmethylpolysiloxane, hydroxy-terminated). respectively. The cyanolipid and triacylglycerol fraction could be identified by a combination of high-temperature gas chromatography with a flame ionization and a nitrogen-phosphorusdetector and gas chromatography-mass spectrometry in positive and negative chemical ionization mode using NH_3 and CH_4/N_2O as reagent gases. The OV-225-OH phase showed better separation properties than the SOP-50 phase and up to 34 peaks could be separated. The analysis of the fatty acids in the seed oil of Allophylus edulis as their methyl esters by high-resolution GC and GC-MS are presented for the first time.