CHIRAL RESOLUTION OF 4-SUBSTITUTED PYRROLIDIN-2-ONES USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ON CELLULOSE AND AMYLOSE CHIRAL STATIONARY PHASES

摘要

Analytical HPLC methods using derivatized cellulose and amylose chiral stationary phases were developed for the separation of the enantiomers of 4-substituted pyrrolidin-2-ones. Separations were performed using normal-phase methodology with a mobile phase of n-hexane-alcohol (ethanol or 2-propanol) in various percentages, and a silica-based cellulose tris-3,5-dimethylphenylcarbamate (Chiralcel OD), or amylose tris-(S)-1-phenylethylcarbamate (Chiralpak AS). The mobile phase and the chiral stationary phase were varied to achieve the best resolution. The effects of alcohol concentration, various aliphatic alcohols in the mobile phase were studied. The effects of substitution were analysed. Baseline separation was easily obtained in many cases.