Protective effects of ebselen on sodium-selenite-induced experimental cataract in rats

摘要

Purpose: To determine whether ebselen has a protective effect or antioxidative potential in a sodium-selenite-induced experimental cataract model. Setting: Firat University, Elazi?, Turkey. Design: Experimental study. Methods: Twenty-one Sprague-Dawley rat pups were randomly divided into a control group, a sodium-selenite-induced-cataract group, and an ebselen-treated group; each group contained 7 rat pups. Rats in the control group received dimethyl sulfoxide (DMSO) intraperitoneally only and rats in the sodium-selenite-induced-cataract group received 30 nmol/g body weight sodium selenite subcutaneously and DMSO intraperitoneally 10 days postpartum. Rats in the ebselen group received 30 nmol/g body weight sodium selenite subcutaneously 10 days postpartum and were treated with 5 mg/kg body weight ebselen once a day for 4 consecutive days. Cataract development was assessed weekly for 3 weeks by slitlamp examination and graded using a scale. Reduced glutathione (GSH), total nitrite, and malondialdehyde (MDA) levels in lens supernatants were measured at the end of 3 weeks. Results: In the control group, all lenses were clear. In the ebselen-treated group, the mean cataract stage was significantly lower than in the sodium-selenite-induced-cataract group (P =.022). The GSH levels were significantly lower in the sodium-selenite-induced-cataract group than in the control and ebselen groups (P <.001). The MDA levels were lower in the ebselen group than in the sodium-selenite-induced-cataract group (P <.001). The mean total nitrite level was significantly lower in the sodium-selenite- induced-cataract group than in the ebselen group (P =.001). Conclusions: Ebselen had a protective effect on cataract development in a sodium-selenite-induced experimental model. The protective effect of ebselen appears to be due to inhibition of oxidative stress. Financial Disclosure: No author has a financial or proprietary interest in any material or method mentioned.