Determination of etoposide inhuman plasma and leukemic cells by high-performance liquid chromatography with electrochemical detection

摘要

This paper describes a high-performance liquid chromatographic method with electrochemical detectionfor the determinationof etoposide levels inplasma, total and non-protein bound concentration, and inleukemic cells. The precision for between-runs (n=6) was 7.0, 4.9, and 9.5%, the accuracy was 3.7, 7.1 and 6.3%, and within-runs precision (n=6) was 3.9, 2.9 and 5.1% for total plasma, non-proteinbound plasma fractionand leukemic cells, respectively. The correlation coefficients (R~2) were 1.00 for all calibrationcurves. These assays have been applied to analyze samples from one patient with acute myelogenous leukemia during 24 h after i.v. infusion of etoposide (100 mg/m~2).