Chromatographic techniques for the determination of putative dietary anticancer compounds in biological fluids

摘要

Although a great number of papers demonstate an association between high intake of fruits and vegetables and reduced risk of certain types of cancer, the epidemiological evidence is not conclusive. The identification adn quantification of sepcific dietary anticancer compounds in plasma, urine and tissues is an important aspect of this research. We surveyed the recet literture for original papers which involved the use of separation techniques for the detection and quantifiction in biological fluids and tissues of putative anticancer compounds which are present in the diet. The compounds included in this review are flavonoids, phytoestrogens. carogenoids, retinoids vitamin E and ascorbic acid. The review covers papers published in the last 3 years. For each class of compounds we discuss the sample preparation , chromatographic conditions, and validation o fthe methods used, in order to identify current trends in the bioanalysis of each class of these substance