High-performance liquid chromatographic analysis of the D_4 receptor antagonist SCH 66712 in rat plasma

摘要

SCH66712 is a potent and selective dopamine D_4 receptor antagonist. An HPLC method was developed for the analysis of SCH 66712 in the plasma of rats,a species used for safety evaluation of this compound. The method involved solid-phase extraction on an ethyl cartridge and HPLC separation on a reversed-phase C_8 column with quantitation using a fluorescence detector. The calibration curve was linear over a concentration range of 5-100 ng/ml. The limit of quantitation was 5 ng/ml,where the coefficient of variation (C.V.) was 2.9% and the bias was 6%. The precision of the method was satisfactory as indicated by an intra-day C.V. of <=4% and an inter-day C.V. of <=6%. The accuracy was also satisfactory as shown by an intra-day bias of <=8% and an inter-day bias of <=9%. The assay was shown to be sensitive,specific,accurate,precise,and reliable for use in pharmacokinetic or toxicokinetic studies.