Determination of the IgE-binding activity of soy lecithin and refined and non-refined soybean oils

摘要

In the present study refined and non-refined soybean oils as well as soy lecithins were investigated for residual allergenicity and compared with extracts from native soybeans.By means of immunoblotting and EAST inhibition experiments no IgE-binding activity was detectable in refined soybean oils,which is probably due to thermal treatment during the refining.The investigated non-refined oils and soy lecithins showed a residual IgE-binding activity.In addition in the lecithin extracts a new IgE-binding structure with a molecular mass of approximately 16 kDa was detectable.