Transient association of the sarcoplasmic reticulum Ca2+ ATPase with the Na+/K+-ATPase and H+/K+-ATPase beta-subunits during its biogenesis in Xenopus oocytes

摘要

We examined the effect of the beta-subunits of the Na+/K+ and H+/K+ ATPases on the biogenesis of the sarcoplasmic reticulum (SR) Ca2+ ATPase in Xenopus oocytes. Oocytes were simultaneously injected with cRNAs for both the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ or the H+/K+ ATPase. Immunoprecipitation with antiserum specific for the beta-subunit of the Na+/K+ or the H+/K+ ATPase yielded not only the respective beta-subunit but also the SR Ca2+ ATPase, indicating that the SR Ca2+ ATPase was associated with the beta-subunits of the Na+/K+ and the H+/K+ ATPases. Pulse-chase experiments revealed that the complex between the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ ATPase was formed transiently and dissociated during the course of maturation. This is the first report that demonstrates the association of the SR Ca2+ ATPase with the beta-subunit of the Na+/K+ and H+/K+ ATPases. [References: 14]