Investigating the Retention Mechanisms of Liquid Ghromatography Using Solid-Phase Extraction Cartridges

摘要

Liquid chromatography (LC), gas chromatography, and capillary electiophoresis are generally considered the most important instrumental separation techniques. While LC separations are based on four retention mechanisms (normal-phase, reverse-phase, ion-exchange, and size-exclusion), experiments for undergraduate laboratory courses often focus on a single retention mechanism, usually reverse-phase. Furthermore, the rationale for the observed retention order is not always apparent, The experiments described below have been used in both first-year BS chemistry major laboratory courses and BA chemistry-biological science major laboratory courses over the past seven years, Collectively, the current experiments have demonstrated an effective, yet inexpensive way for students to investigate LC retention mechanisms.