Stability and catalytic properties of lipase immobilized on chitosan encapsulated magnetic nanoparticles cross-linked with genipin and glutaraldehyde

摘要

BACKGROUNDAmong many cross-linkers for enzyme immobilization, genipin (Gen) and glutaraldehyde (Glu) as cross-linkers have been commonly investigated to improve the catalytic performance of enzyme immobilized on chitosan encapsulated magnetic nanoparticles (CMNPs). In this work, we compared the stability and catalytic properties of lipase Candida rugosa (CRL) immobilized on CMNPs cross-linked with Gen and Glu, respectively. The CMNPs were first characterized by TEM, XRD and FT-IR. Moreover, some crucial parameters affecting catalytic performances were optimized for Gen-CMNPs-CRL and Glu-CMNPs-CRL preparation.