Whole cell bioconversion of (+)-valencene to (+)-nootkatone by Yarrowia lipolytica using a three phase partitioning bioreactor

摘要

BACKGROUNDLow permeability of substrates across the cell membrane, cofactor regeneration and product inhibition are some drawbacks of (+)-nootkatone bioconversion. The aim of this work was to evaluate and enhance the bioconversion of (+)-valencene to (+)-nootkatone with Yarrowia lipolytica in a partitioning bioreactor using orange essential oil as the dispersed phase.