Adsorption and Activity of Papain on Hierarchically Nanoporous Silica

摘要

Hierarchically nanoporous silica SBA-1 (HSBA-1) particles were successfully fabricated by employing organic mesomorphous complexes of poly(methyl vinyl ether-a/t-maleic acid） (P(MVE-a/t-MA)) polyelectrolyte and cationic surfactant (hexadecyl pyridinium chloride (CPC)) as templates. The synthesized HSBA-1 possessed ordered mesopore of 2.9 nm and nanopores of 27 nm with well-defined morphologies and well-ordered cubic Pm3n mesostructure. The application of HSBA-1 as a scaffold for papain immobilization was studied. It was found that ascribed to the hierarchical pore structure the adsorption equilibrium time was low (2 h) and the optimum adsorption pH value was 8.5. The activity of the immobilized papain was examined. Compared with free enzyme, the immobilized papain showed improved thermastability and pH stability.