Treatment with hydrogen molecules prevents RANKL-induced osteoclast differentiation associated with inhibition of ROS formation and inactivation of MAPK, AKT and NF-kappa B pathways in murine RAW264.7 cells

摘要

The bone protective effects of the hydrogen molecule (H_2) have been demonstrated in several osteoporosis models while the underlying molecular mechanism has remained unclear. Osteoclast differentiation is an important factor related to the pathogenesis of bone-loss related diseases. In this work, we evaluated the effects of incubation with H_2 on receptor activator of NFkB ligand (RANKL)-induced osteoclast differentiation. We found that treatment with H_2 prevented RANKL-induced osteoclast differentiation in RAW264.7 cells and BMMs. Treatment with H_2 inhibits the ability to form resorption pits of BMMs stimulated by RANKL. Treatment with H_2 reduced mRNA levels of osteoclast-specific markers including tartrate resistant acid phosphatase, calcitonin receptor, cathepsin K, metalloproteinase-9, carbonic anhydrase typell, and vacuolar-type H~+-ATPase.