Enhanced Gene Delivery Using Biodegradable Poly(ester amine)s (PEAs) Based on Low-Molecular-Weight Polyethylenimine and Poly(e-caprolactone)-Pluronic-Poly(e-caprolactone)

摘要

In this paper, the poly(ester amine)s (PEAs) were successfully prepared from low-molecular-weight PEI (Mn = 2000) and Poly(s-caprolactone)-poly(ethylene glycol)–poly(propylene glycol)– poly(ethylene glycol)–poly(ε-caprolactone) (PCFC) copolymers using isophorone diisocyanate (IPDI) as cross-linker. The obtained PEAs copolymers are biodegradable and water-soluble. The PEAs/DNA complexes showed effective and stable DNA condensation with the particle size <200 nm and zeta potential >10 mV, indicating its potential for intracellular delivery. Compared to the unmodified low-molecular-weight PEI, PEAs displayed similarly low cytotoxicity in all two cell lines (293T: Human kidney carcinoma, HUVEC: Human umbilical vein Endothelial cell) and revealed much higher transfection efficiency in 293T cell lines. Therefore these PEAs might be a novel safe and efficient polymeric gene delivery vectors.