Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of ade1 or ade2 mutants

摘要

We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1 -methyl-3-nitro-l -nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met 30 strains carrying adel and aae2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry.