A Modified Coomassie Brilliant Blue G 250 Staining Method for the Detection of Chitinase Activity and Molecular Weight after Polyacrylamide Gel Electrophoresis

摘要

A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis(PAGE)is presented.The staining formed achromatic zones at the locations of the migrated enzyme.Using Streptomyces griseus chitinase,we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.