embedded image "/> Polyacrylamide gel for electrophoresis, polyacrylamide gel electrophoresis method using the same, method of producing the same, and acrylamide compound
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Polyacrylamide gel for electrophoresis, polyacrylamide gel electrophoresis method using the same, method of producing the same, and acrylamide compound

机译:电泳用聚丙烯酰胺凝胶,使用其的聚丙烯酰胺凝胶电泳方法,其制造方法以及丙烯酰胺化合物

摘要

It is intended to provide a method for easily detecting a phosphorylated peptide (protein) in a test sample by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which has been conventionally employed in analyzing a protein; a polyacrylamide gel for electrophoresis to be used in the method; a method of producing the gel; and a synthesizing intermediate in producing the gel. The polyacrylamide gel for electrophoresis to be used in the method of the invention is characterized in that at least a part of the structure thereof has a structure represented by the following formula (I); wherein M2+represents a transition metal ion; and X represents a linker group.; embedded image
机译:本发明旨在提供一种通过使用常规用于分析蛋白质的SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)容易地检测测试样品中的磷酸化肽(蛋白质)的方法。该方法中使用的用于电泳的聚丙烯酰胺凝胶;生产凝胶的方法;以及制备凝胶的合成中间体。本发明的方法中使用的电泳用聚丙烯酰胺凝胶的特征在于,其结构的至少一部分具有下式(I)表示的结构。其中M 2 + 表示过渡金属离子; X表示连接基。 “嵌入式图像”

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