Expression of Aspergillus oryzae Phytase Gene in Aspergillus oryzae RIB40 niaD~-

摘要

Aspergillus oryzae RIB40 niaD~-was transformed using a plasmid constructed with the A.oryzae phytase gene and pNAN8142 vector.The culture broth of the transformant,which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml~(-1) at 37 deg C,pH 5.5.