Analyses of Microbial Community within a Composter Operated Using Household Garbage with Special Reference to the Addition of Soybean Oil

摘要

A commercially available composter was operated using fixed composition of garbage with or without the addition of soybean oil. The composter was operated without adding seed microorganisms or bulking materials. Microflora within the composter were analyzed by deaturing gradientg gel electrophoresis (DGGE) in the case of oil addition, or by 16/18S rRNA gene sequencing of the isolated microorganisms in the case of no oil addition. The results showed that, irrespective of the addition of oil, the bacteria identified were all gram positive, and that lactobacilli seemed to be the key microorganisms. Based on the results, suitable microflora for use in a household composoter are discussed.