Functional Investigation of Effects on Transplantable Bone Marrow Stem Cells Label with Iron Oxide Nanoparticles

摘要

Noninvasive techniques tracking the transplanted stem cells played an important role in regenerative medicine and it remains significant to investigate the interference of contrast agents to the growth and differentiation of stem cells. The study investigate the effects of superparamagnetic iron oxide particles (SPIO) labeling on the rat bone marrow mesenchymal stem cells (BMSCs) and evaluate the feasibility of in vivo tracking with noninvasive MRI. Commercial Resovist was added to culture at different concentration equivalent to 14, 28, 56,112 mu g Fe/mL and without the contrast agents in the control group. The cytoxicity and labeling efficiency of SPIO labeling-BMSCs were determinate by MU assay and Prussian Blue staining, respectively. BMSCs of all groups were induced by DMSO and BHA for six hours, subsequently, the mRNA expressions of neuroepithelial stem cell protein (Nestin), neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP), as well as nestin protein expression of BMSCs were examined by RT-PCT and western blot technique after differentiation. Compared with the control group, the expression of nestin, NSE and GFAP was positive in groups of different concentrations and without statistical significance, as well as nestin protein expression (P < 0.05). Besides, we used susceptibility weighted imaging (SWI) to demonstrate the low signal intensity of BMSCs labeled by SPIO. Our results showed that efficient labeling of BMSCs at an optimized dosage of SPIO nanoparticles didn't affect the growth and differentiation properties of BMSCs. Simultaneously, the process of differentiation of SPIO labeled BMSCs can be detect in vivo by MRI which demonstrate that SPIO nanoparticles may enable noninvasive in vivo tracking of BMSCs in experimental and clinical settings during cell-based therapies.