Design and Evaluation of a Cell Microencapsulating Device for Cell Assembly Technology

摘要

A cell encapsulation device based on the principle of high-voltage dispersion technology was designed and constructed. The parameters that influenced the formation of cell microcapsules, such as; voltage, push speed, electrode distance, and syringe size were analyzed and optimized. Unlike the traditional cell microencapsulating devices, both of the electrodes were separated from the CaCl2 solution in this device, which simplified the procedure of keeping the operation within a sanitary environment. Integrated adipose derived stem cell (ADSC) microcapsules with smooth surface and consistent diameter distribution were prepared. After 1-week in culture as a microcapsule and 3 days assembled in a construct, the ADSCs maintained a high viability and significant proliferation that established a favorable basis for the controlled assembly of cell microcapsules.