Collagen/Chitosan/Heparin Complex with Improved Biocompatibility for Hepatic Tissue Engineering

摘要

To make an implantable bioartificial liver(IBL),a new biocom-patible collagen/chitosan/heparin complex was prepared using a crosslinking agent.The X-ray photoelectron spectroscopy(XPS),mechanical strength and biocompatibility with whole blood and hepatocytes were measured.The collagen/chitosan/heparin complex resulted in a superior blood compatibility compared to l-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC)crosslinked collagen matrix.The morphology and behavior of the cells on the collagen/chitosan/heparin membrane were found to be different from those on the collagen and collagen/chitosan membranes.Cells on the collagen membrane formed smaller three-dimensional aggregates than those on the collagen/chitosan membrane,while on the collagen/chitosan/heparin membrane,a round shape with no junctions were manifested.No adverse effects were found on the viability and function of the hepatocytes on the collagen/chitosan/heparin membrane compared to the collagen and collagen/chitosan membranes.These results suggest that this collagen/chitosan/heparin matrix is a potential candidate for hepatic tissue engineering.