Bone in Growth in bFGF Versus BMP Combined with bFGF Saturated PLA-PEG-PLA:Experiment in Surgically Created Rabbit Mandibular Defects

摘要

A triblock polymer of LPLA and PEG 1000 and 3400 at 3% and 9% was formed by ring opening under nitrogen atmosphere at 120 deg C and then,in one group,bFGF was added,while in the other both bFGF and BMP were added,leaving one group as a control.Gross,radiographic,histological and scanning electronic microscope observations were done.With the help of motic images advanced 3.0 new bone formed,calculated as a percentage of total selected area and,using SAS6.12,results were analyzed.Statistical analysis between groups showed that,at 2 weeks,the PLA-PEG-PLA+bFGF+BMP group had the highest amount of new bone formation,as a percentage of new bone over total surface area,at 48% followed by the PLA-PEG-PLA+bFGF group at 39%,then the PLA-PEG-PLA=BMP group at 29%.The PLA-PEG-PLA group trailed in at 18% (statistical significance,p < 0.05=0.0001).After 4 weeks,the experimental groups B and D had almost equal new bone formation (62% and 64% respectively),but still significantly different from the control group and BMP2 group,with 43% and 38% respectively (p < 0.05=0.0001).After 8 weeks,there was no difference in the amount of bone regeneration (p> 0.05=0.87).Within the groups,the control group exhibited steady incremental new bone formation at 2,4 and 8 weeks,starting out at 18%,followed by 42% and lastly 65%.Interestingly this phenomenon also applied to group C (BMP2 group) but not to the B group (bFGF group).The differences at the respective consecutive time of examination were statistically significant (p<.0.05=0.0001).However,in both experimental groups B and D there was statistically significant difference between new bone formed at 2 weeks compared to that at 4 weeks,but thereafter the increment was negligible.