Isozymic typing for identification of sesbania accessions

摘要

A set of 40 accessions of 13 Sesbania species were investigated for six isozyme systems (acid phosphatase, peroxidase, amylase, catalase, malate dehydrogenase and glutamate dehydrogenase) using polyacrylamide gel electrophoresis (PAGE). A total of 193bands, all polymorphic were observed over 6 isozymic systems. Malate dehydrogenase was the most polymorphic, distinguishing 38 accessions unambiguously with 38 unique patterns. This was followed by glutamate dehydrogenase and acid phosphatase that couldcharacterize 31 accessions distinctly with their 31 unique banding patterns. Amylase exhibited 25 unique banding patterns while peroxidase and catalase had 23 unique banding patterns. None of the enzyme system could alone distinguish all the accessionsindividually. However, a combination of any of the two enzyme systems may be used to distinguish all 40 accessions uniquely. Results of present study suggest that sufficient variability exists in Sesbania to allow the use of isozyme analysis as a systemfor accession characterization, thus complementing the traditional methods currently being used to publish descriptors of accessions.